Fig. 5.

Endosomal NOX2 oxidase suppresses cytokine expression in response to TLR7 activation in vitro and in vivo. a, b WT and TLR7^−/− immortalized bone marrow-derived macrophages (BMDMs) were untreated or treated with imiquimod (Imiq; 10 μg/ml) in the absence or presence of a apocynin (Apo; 300 μM) or b bafilomycin A (Baf-A; 100 nM), and IFN-β, IL-1β, TNF-α and IL-6 mRNA expression determined by QPCR after 24 h (n = 6). c, d WT and NOX2^−/y mice were administered with imiquimod (50 μg per mouse, intranasal) and c total airway inflammation quantified by bronchoalveolar lavage fluid analysis and d cytokine expression assessed 24 h later (n = 5). a, b, d Responses are relative to GAPDH and then expressed as a fold-change above WT controls. a–d Data are represented as mean ± S.E.M. a, b and d Kruskal–Wallis test with Dunn’s post hoc for multiple comparisons. c One-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons. Statistical significance was accepted when P < 0.05. *P < 0.05; **P < 0.01