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. 2017 Jul 12;7:5169. doi: 10.1038/s41598-017-04569-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of proliferative capacity in primary corneal epithelial cultures between limbal and central cornea in mouse and human. (A) Phase-contrast microscopy showed the mouse epithelial cell growth of explants from limbal and central corneas on days 3, 5 and 7; (B) Human epithelial cell growth of explants from limbal and central corneas on days 3, 7 and 14. (C) WST-1 assay showed no difference of epithelial cell growth between limbal and central corneal explants from mice during 7 days (n = 5, P > 0.05); but there is growth difference between limbal and central corneal explants of human during 14 days. Data were summarized from 5 separated experiments. *P < 0.05 and ***P < 0.001, as two groups compared. (D) The representative images from clonogenic assays of the single cells from corneal or limbal epithelia in mice and human.