Figure 4.

Bacillus Calmette–Guérin (BCG) engagement of multiple toll-like receptors (TLRs) and C-type lectin-like receptors accounts for early release of induced cytokines. (A–C) Concentrations of selected cytokines measured by Luminex assay from purified CD14⁺ monocytes [(A), n = 5–8], CD66b⁺ neutrophils [(B), n = 8)], and CD2⁺ lymphocytes [(C), n = 8] each stimulated with BCG in the presence of indicated neutralizing antibodies (black circles) or isotype control antibodies (red circles) or neutralizing anti-TLR5 (white circle), for 5 h (monocytes and neutrophils) or 16 h (lymphocytes). Filled circles indicate the median concentration for each condition, and the lines represent the interquartile (IQR1/3) range. The dotted lines indicate the respective median values for unstimulated samples; if not shown, the null condition was below the limit of detection for the given assay. (D) Heat map representation using the median concentration determined from data represented above. p Values were determined by the paired Student’s t test and false discovery rate corrected for multiple analyte testing. *q ≤ 0.05; **q ≤ 0.01; ***q ≤ 0.001 as compared to BCG + isotype control antibody.