Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 12;7:5230. doi: 10.1038/s41598-017-04657-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

A candidate-based BAC screen identifies new genetic tools for targeting neuronal subpopulations. (a) Workflow to establish BAC transgenic fish lines. WT, wild-type. (b) Plasmid constructs used for BAC recombineering. KanR, kanamycine-resistant gene; pA, polyadenylation signal. (c) Relationship between the genomic insert length of all BAC constructs (7 Gal4VP16 BACs, 39 Gal4VP16-Bleeding Heart BACs, and 12 Cre-Cold Heart BACs) and their founder rate (percentage of germline founders out of total adult fish screened). Note that the founder rate is not correlated with the genomic insert length (R-squared = 0.0007). (d) Dorsal view of 6 dpf old larval brains showing the live expression pattern of ten selected transgenic Gal4 lines (green; Dendra-kras, GCaMP6s or EGFP). Brains have been registered via co-expression of HuC:lynTagRFP-T (magenta). Scale bar, 100 µm.