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. 2017 Jul 12;7:5230. doi: 10.1038/s41598-017-04657-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Registration of transgenic lines illustrates expression in distinct neuronal subpopulations. (a) Dorsal view on a reference brain showing the expression of selected, co-registered Gal4 lines. (b,c) Z-projection of confocal stacks showing the expression pattern of chat:Gal4, gad1b:Gal4, sst3:Gal4 and galn:Gal4 or cxcr4b:Gal4, respectively, in the deep fore-/midbrain (b), and in the hindbrain (c). (d–f) Single confocal slices of chat:Gal4, gad1b:Gal4, and cxcr4b:Gal4 expression in the hindbrain, in dorsal (d), lateral (e) and cross-sectional view (f). Dashed lines indicate plane positions. Scale bar, 100 µm in (a) and 50 µm in (b–f).