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. 2017 Jul 12;7:5230. doi: 10.1038/s41598-017-04657-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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New transgenic lines label distinct sublaminae in the tectal neuropil. Lateral view of the tectal neuropil shows registered expression pattern of chat:Gal4, cxcr4b:Gal4, dmbx1b:Gal4, gad1b:Gal4, and isl2b:Gal4, as merged (a) or single channels (c–f). (b) Fluorescence intensity plots along the boxed regions in (a–f). Intensity peaks of isl2b:Gal4 expression were used for layer determination. SIN cell bodies labeled by gad1b:Gal4 are marked by arrowheads in (e). The peak for dmbx1b:Gal4 in the SPV layer reflects labeled periventricular cell bodies.