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. 2017 Jul 13;8:486. doi: 10.3389/fphys.2017.00486

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Copyright © 2017 Chen, Orriols, Walther, Laghmani, Hoogeboom, Hogen-Esch, Hiemstra, Folkerts, Goumans, ten Dijke, Morrell and Wagenaar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA expression of bone morphogenetic protein 9 (BMP9; A) activin receptor-like kinase 1 (ALK1; B) activin receptor-like kinase 2 (ALK2; C) BMP type II receptor (BMPRII; D) Endoglin (E) and Transmembrane protein 100 (TMEM100; F) in lung of rat pups on days 1, 3, 6, and 10 after birth and in adult rats during normal development in RA (white bars) and after exposure to 100% O₂ (shaded bars), using β-actin as a house keeping gene reference. Values are expressed as mean ± SEM (N = 6–10). Differences between groups were analyzed by one-way ANOVA, followed by Tukey's multiple comparisons test. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 vs. own age-matched RA controls. ^ΔΔp < 0.01 and ^ΔΔΔp < 0.001 vs. RA control on day 1.