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. 2017 Jul 13;8:486. doi: 10.3389/fphys.2017.00486

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Copyright © 2017 Chen, Orriols, Walther, Laghmani, Hoogeboom, Hogen-Esch, Hiemstra, Folkerts, Goumans, ten Dijke, Morrell and Wagenaar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA expression of BMPRII, ALK1, ALK2, and TMEM100 in human primary pulmonary arterial endothelial cells (PAEC), human microvascular endothelial cell line (HMEC), human primary bronchial epithelial cells (PBEC), and human epithelial pulmonary mucoepidermoid carcinoma cell line (NCI-H292) (A). Relative mRNA expression of inhibitor of DNA binding 1 (ID1) in endothelial cells (PAEC and HMEC) and epithelial cells (PBEC and NCI-H292) treated with 5 ng/ml of BMP9 for 4 h (B). Relative mRNA expression of MCP-1 (C), IL6 (D), and TMEM100 (E) in endothelial cells (PAEC and HMEC) treated with BMP9 for 4 h. Values are expressed as mean ± SEM. ^***p < 0.001 vs. Control using acidic ribosomal protein (ARP) as a reference gene.