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. 2017 Jul 13;8:1328. doi: 10.3389/fmicb.2017.01328

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Copyright © 2017 Blötz, Treffon, Kaever, Schwede, Hammer and Stülke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of c-di-AMP binding proteins. Detection of in vitro c-di-AMP binding was performed with c-di-AMP immobilized to an agarose matrix. This was incubated with cell extract from M. pneumoniae. The c-di-AMP bound proteins were eluted with free c-di-AMP and analyzed by SDS-polyacrylamide gel electrophoresis and visualized by silver staining. Proteins were identified by mass spectrometry. Arrows show mass spectrometry analyzed bands. E, elution fraction; +, agarose coupled with c-di-AMP; -, agarose coupled with ethanolamine.