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. 2017 Jul 13;7:299. doi: 10.3389/fcimb.2017.00299

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Copyright © 2017 Shahid, Wang, Gu, Chen, Ali, Gao, Han, Yang, Fanning and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adhesion of P. zopfii and its effect on bMECs morphology at different time of infection. (A) control, (B,C) 4 h, (D,E) 12 h, and (F,G) 24 h. Black arrowheads show the type-II, while white arrowheads show type-I cells adhesion. (B,C,E,F) There were no significant changes in bMECs, (D) Depicting slight cells morphological changes in type-II at 12 h, (G) Showing disruption of monolayer of bMECs in type-II at 24 h. (H) Time course of the survival and adhesive capability of P. zopfii in bMECs that increased significantly with passage of time. Data indicated as Mean ± SD of three independent experiments. ^**p < 0.01.