FIGURE 3.

Expression of the cheA gene in the wild-type and ΔtlpA1 strains. (A) The cheA and tlpA1 genes were detected by PCR using the WT and ΔtlpA1 cDNA as templates; 16S rRNA gene was amplified as a positive control. (B) Expression of the cheA gene in the WT and ΔtlpA1 strains was detected by qRT-PCR, with 16S rRNA gene as internal standard. All data were derived from three independent experiments and are expressed as mean and SD. The WT and ΔtlpA1 strains were grown in TY medium (OD₆₀₀ = 0.8).