Figure 1.

Specificity of the SNAP47 antibody confirmed by the western blot of cultured WT hippocampal neurons infected with lentivirus expressing GFP-tagged mouse or rat SNAP47.Cultured WT hippocampal neurons were infected with either GFP (left lanes), GFP-mouse-SNAP47 (middle) or GFP-rat-SNAP47 (right) expressing lentiviral particles. From these cultures, 30 μg of protein lysates were loaded per lane and subjected to western blotting. The blot on the left was incubated with the rabbit polyclonal anti-SNAP47 antibody, the blot on the right was incubated with an anti-GFP antibody. Synaptophysin (~37 kDa) expression served as internal loading control detected by an anti-synaptophysin antibody. Note that the anti-SNAP47 antibody specifically recognizes the mouse or rat GFP tagged SNAP47 (~74 kDa) as well as the endogenously expressed SNAP47 protein (47 kDa). The anti-GFP antibody in the western blot on the right detects the expression of GFP (~27 kDa) and the GFP-tagged SNAP47 but not the endogenous SNAP47.