Table 1.
List of primary antibodies for immunocytochemistry.
| Antibody | Supplier and cat. no. | Host | Dilution | Immunogen | |
|---|---|---|---|---|---|
| SNARE protein | |||||
| SNAP47 | Synaptic System | Rabbit | LM 1:300 | Recombinant full length rat SNAP47. | |
| 111 403 | (polyclonal, affinity purified) | EM 1:30 | |||
| Pre-synaptic glutamatergic marker proteins | |||||
| ZnT3 | Synaptic System | Guinea pig | LM 1:300 | Recombinant protein of mouse ZnT3. | |
| 197 004 | (polyclonal, crude antiserum) | (aa 2–75). | |||
| VGLUT1 | Synaptic System | Guinea pig | LM 1:2000 | Purified recombinant protein of rat | |
| 135 304 | (polyclonal, crude antiserum) | VGLUT1 (aa 456–560). | |||
| Post-synaptic glutamatergic marker protein | |||||
| PSD95 | UC Davis/NIH | Clone K28/43 | 1:100 | Fusion protein amino acids 77–299 | |
| NeuroMab Facility | Mouse | (PDZ domains 1 and 2 of human PSD95). | |||
| 75–028 | (monoclonal) | ||||
| GFP/YFP | |||||
| GFP | UC Davis/NIH | Clone N86/38 | LM 1:2000 | Green fluorescent protein against fusion protein amino acids 1–238 (full length) of jellyfish green fluorescent protein. | |
| NeuroMab Facility | Mouse | EM 1:100 | |||
| 75–132 | (monoclonal) |