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. 2017 Jul 13;8:1235. doi: 10.3389/fpls.2017.01235

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Copyright © 2017 Colas, Darrier, Arrieta, Mittmann, Ramsay, Sourdille and Waugh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ASY1 re-organization during desynapsis and chromosome segregation. Strong ASY1 signal are detected after prophase I during chromosome segregation in both wild type (a–f) and des10 (g–m). Discrete ASY1 foci (green) are visible in wild type diplotene (a). At metaphase I, ASY1 signal does not seems specific and is located in the cytoplasm (b). During anaphase I, ASY1 signal is either linear (c) or globular (d,e). Discrete ASY1 signals are also found in tetrads (f). ASY1 behaves the same in des10, but the delay in prophase enables more details to be studied. ASY1 signal is present on diplotene chromosomes (g), and it is possible to visualize the last ASY1 signal near the end of the chromosomes (h, arrow). During metaphase I, ASY1 is mainly in the cytoplasm (I) but during anaphase I, it is possible to see the ASY1 signal relocated around the lagging chromosomes (k,l arrows). Discrete ASY1 signal is also found in tetrads (m). Scale bar 5 μm.