Table 1.

Standard solutions composition.

Reactive	Standard solutions (mM)
	Krebs’	C-clamp Bath	C-clamp Electrode	ICa2+ Bath	ICa2+ Electrode	Potassium currents
NaHCO3	14.4
NaH2PO4	1.35
NaCl	120	130
Glucose	12.7	5		5		1
KCl	6	5	30			5
CaCl2	2.5	2	2			1
MgCl2	1.35	1	1	2	4	1
HEPES		10	10	10	10	2
K-Gluconate			110
NMDG				95		140
TEA-Cl				35
BaCl2				10
4-AP				1
Cs-Gluconate					130
EGTA					10
ATP-Mg					4
GTP-Na					0.3–0.5
CdCl2						0.1
pH		7.3–7.4 NaOH	7.25 KOH	7.3–7.4 HCl	7.25 CsOH	7.3–7.4 HCl