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. 2017 Jul 12;7:5178. doi: 10.1038/s41598-017-05461-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Contour density plots for determination of fractions of early apoptotic, late apoptotic, and necrotic cell death of live WBC cells, respectively. Panel A shows the untreated cells as control, while (B)displays the treated cells with LD₅₀ concentrations of ASNPs, for 24 h. The cell necrosis and apoptosis were measured by using 7-AAD and Annexin-V dyes in flow cytometry based assays.