Figure 2. Effects of treadmill exercise on the level of Aβ-42 in skeletal muscle of NSE/PS2m mice. The treadmill exercise reduced the levels of Aβ-42 expression and distribution of red Aβ deposition in the plantaris muscle of PS2-Tg mice. (A) Relative levels of Aβ-42 were analyzed in triplicate by western blot. The band intensity of Aβ-42 expression was normalized to GAPDH levels as an internal control. The values were converted to percentage of the non-Tg Con group (each value represents mean ± SD, n=5/group). Data were analyzed using a two-way ANOVA followed by Fisher’s LSD post-hoc test (***P < 0.001). (B) Immunostaining of Aβ deposition in the plantaris muscle, which was assayed using Congo red staining, was seen in Tg mice (see red arrows), but not in non-Tg mice.