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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Nat Microbiol. 2017 Jan 9;2:16251. doi: 10.1038/nmicrobiol.2016.251

Figure 5. Genetics of Cluster N prophage-mediated defenses.

Figure 5

(a–e) Ten-fold serial dilutions of phage lysates were spotted onto lawns of M. smegmatis mc2155, lysogens and lysogens of mutant phage derivatives, as indicated beneath each panel. Phage names are shown to the left, including mutants (e.g. PhrannΔ29) and Defense Escape Mutant (DEM) derivatives of Tweety that overcome Phrann-mediated defense. All platings were performed in at least two separate experiments. (f) Bam HI restriction digestion of DEM DNAs shows changes in a 2.4 kbp fragment containing gene 54 (arrow). Marker (M) fragments are shown in kbp. (g) ClustalX alignment of gp54 of Tweety and DEM mutants. The wild type Tweety genome was re-sequenced and 54 contains 39 repeat units, rather than 48 in the original sequence 32; repeat consensus: AAXX, where XX is either GS, GY, WS, WY, QS, or QY) flanked by unique sequences.