Skip to main content
. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Methods Mol Biol. 2017;1486:357–390. doi: 10.1007/978-1-4939-6421-5_14

Fig. 2.

Fig. 2

Enzymatic protein biotinylation. (A) Diagram showing the biotinylation reaction catalyzed by BirA biotin ligase. It recognizes the 15 amino acid AviTag (red) and conjugates biotin to the lysine residue in the presence of ATP. (B) SDS gel electrophoresis to test the yield of protein biotinylation.