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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Methods Mol Biol. 2017;1486:357–390. doi: 10.1007/978-1-4939-6421-5_14

Fig. 4.

Fig. 4

Microfluidic chamber used in optical trapping experiments. (A) Microfluidic chamber installed on a motorized translational stage and positioned between two objectives. The PE tubing connected to the three channels and to the protein tube can be seen. (B) Microfluidic chamber installed on the chamber frame. (C) Microfluidic chamber showing three channels, two dispenser tubes, and the protein tube. The thickness of channels between two coverslips is around 180 μm. (D) Diagram illustrating how the microfluidic channel is assembled onto the frame. The figure is reproduced from our earlier work with permission (41).