Skip to main content
. 2017 Jul 10;8:15999. doi: 10.1038/ncomms15999

Figure 3. Sequencing of primary sarcomas generated using a combined CRISPR-Cas9-Cre-loxP system.

Figure 3

(a) Sanger sequencing of the Trp53 gene in tumours generated in KC mice after adenoviral delivery of pX333-sgTrp53-Cre. Most of the sequenced alleles demonstrated deletions but there was a single nucleotide insertion observed in mouse 2981- allele 1. (b) Targeted deep sequencing of the Trp53 gene in tumours generated in KC mice. (Blue=sgTrp53; green=PAM; red=insertion/deletion). (c) Sanger sequencing of the on-target chromosome 17 site in tumours generated in KC mice after adenoviral delivery of pX333-sgTrp53-Cre. Most of the sequenced alleles demonstrated deletions but there was a single nucleotide insertion observed in mouse 2981- allele 1 and two nucleotides insertion observed in mouse 2995 allele 2. (d) Targeted deep sequencing of the on-target chromosome 17 site in tumours generated in KC mice. (Blue=sgTrp53; green=PAM; red=insertion/deletion).