Figure 5. CRISPR-Cas9-mediated generation of primary MPNSTs.

(a) The px333 plasmid was modified by cloning a sgRNA targeting Nf1 into the plasmid following the first U6 promoter and a sgRNA targeting Trp53 following the second U6 promoter. (b) Wild-type 129/SvJ mice received sciatic nerve injections of an adenovirus carrying CRISPR components, and the mice were monitored for tumour formation. The formation of tumours was observed at the site of injection in these wild-type mice and (c) Surveyor analysis revealed indels in Nf1 and Trp53. (d) Haematoxylin and eosin histology and (e) positive S100 staining were consistent with MPNST. Scale bars, 100 μm.