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. 2017 Jul 10;8:16044. doi: 10.1038/ncomms16044

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Schematic of endogenous PV1, PV2 and EXP3 proteins tagged with HA and glmS. Sizes of fusion proteins are indicated. All proteins contain predicted signal peptides (SPs) and EXP3 contains a predicted transmembrane (TM) domain. Numbers refer to amino acids. (b) Western blots were probed with anti-HA antisera to confirm PV1-HA, PV2-HA and EXP3-HA fusion protein expression in transfectants. Expected full-length fusion proteins are marked by arrows. Molecular masses are shown in kDa. (c) Immunofluorescence analysis of PV1-HA-, PV2-HA- and EXP3-HA-infected RBCs labelled with antisera recognizing HA (green) and PV1 (red). DIC and DAPI (blue) are shown on the left. Scale bars, 5 μm.