Fig. 5.

CXCR3A, but not CXCR3B, stimulation with CXCL11 causes a change in βarr2 conformation (A). Schematic of βarr2 biosensor for probing βarrestin-conformational changes following transfection with the biosensor and untagged CXCR3A or CXCR3B. HEK 293 cells were stimulated for 15 minutes with the indicated ligand (250 nM) prior to BRET measurements. (B) CXCL11 stimulation of CXCR3A led to a significant conformational change in βarrestin signified by a change in magnitude of the net BRET ratio compared with all ligands, while CXCL10 stimulation caused a significantly different signal from CXCL4. (C) Stimulation of CXCR3B resulted in no appreciable change in signal across all comparisons. *P < 0.05 by one-way analysis of variance with Tukey’s post hoc comparison between all treatment groups. n ≥ 3 biologic replicates per condition. n.s, not significant.