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. 2017 Jul 11;8:15957. doi: 10.1038/ncomms15957

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The 0.1 μM FtsA*, showing short straight polymeric structures; (b) 0.5 μM FtsA*, showing mostly arcs and short curved protofilaments. Inset shows × 2.5 magnification of a portion of the image, highlighting some FtsA* arcs (scale bar in inset, 40 nm). (c) The 0.1 μM or (d) 0.5 μM FtsA*+5 μM FtsZ, showing bundling of FtsZ protofilaments (large arrow) and FtsA* arcs (smaller arrows). (e) The 0.5 μM FtsA*+ 5 μM FtsZ*, showing assembly of very large FtsZ protofilament bundles/sheets (arrow). (f,g) The 0.5 μM FtsA+5 μM FtsZ*, showing persistence of FtsZ* bundles in the presence of FtsA minirings. (h) Same as (f,g) except with 2.5 μM FtsZ*. Arrows highlight FtsZ* protofilament bundles. (i) Mock experiment with FtsZ* on monolayers, but no FtsA added, showing an unusually dense area with FtsZ* polymers on the monolayer for comparison. Scale bar, 100 nm.