pLTF positively correlates with serum E2 levels, and inversely correlates with the P/E ratio. A, Serum E2 levels were significantly elevated in proestrus (PRO) and OVX+E rats relative to estrus (EST) and OVX rats. B, Regression analyses of serum E2 levels, which positively correlated with the magnitude of pLTF 60 min after aIH. C, Serum progesterone levels were reduced in OVX and OVX+E rats. D, Serum progesterone levels did not correlate with pLTF. E, The P/E ratios in the PRO and OVX+E groups were significantly lower than P/E ratios in the EST and OVX groups. F, The P/E ratio negatively correlated with pLTF. ***p < 0.001 and *p < 0.05 versus PRO; †††p ≤ 0.001 versus OVX+E; ‡p < 0.05 versus EST. Overlaid box plots indicate the 25th and 75th percentile, and the median of each dataset; group means are denoted by bold lines.