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. 2017 Jun 12;9(1):1320193. doi: 10.1080/20002297.2017.1320193

Figure 3.

Figure 3.

Invasion of F. nucleatum is suppressed by PI3K inhibition. (A) HOK-16B cells were preincubated with wortmannin at the indicated concentration for 30 min and then infected with CFSE-labeled F. nucleatum and unlabeled wild-type P. gingivalis at a MOI of 500 for 4 h. The percentage of cells containing F. nucleatum (left panel) and the MFI (right panel) are shown as the mean ± standard deviation. *p < 0.05 compared with cells without preincubation with wortmannin in F. nucleatum monoinfection; †p < 0.05 compared with cells without preincubation with wortmannin in mixed infection with F. nucleatum and P. gingivalis; #p < 0.05 compared between the two groups. (B) HOK-16B cells were preincubated with 5 μM of wortmannin for 30 min and infected with CFSE-labeled F. nucleatum and unlabeled wild-type P. gingivalis or gingipain mutants at a MOI of 500 for 4 h. *p < 0.05 compared with F. nucleatum monoinfection in the absence of wortmannin; †p < 0.05 compared with F. nucleatum monoinfection in the presence of wortmannin; #p < 0.05 compared between the two groups. Representative data from three independent experiments are shown. Fn, F. nucleatum; Pg, P. gingivalis; WT, P. gingivalis ATCC 33277; 129, KDP129 (kgp−); 133, KDP133 (rgpA− rgpB−); 136, KDP136 (kgp− rgpA− rgpB−).