Table I.

Sequences of the primers utilized for nested polymerase chain reaction (PCR)

Species	Sequence	Primer	Length
TTSuV1a	5′-AACTGGCAGGACCACCTATG-3′	Forward	481 bp
	5′-AGTGTBACHTCHCCACTYC-3′	Reverse
	5′-AAAGAGACGCTATGGCTGGA-3′	Forward nested	255 bp
	5′-TGYTTTTCWGTGTCCCAYTGC-3′	Reverse nested
TTSuV1b	5′-ATGCCTTACAGACGCTATC-3′	Forward	605 bp
	5′-TGTGATGTTAATTTTGGTGGA-3′	Reverse
	5′-AAGCTCCGGTCATACAATG-3′	Forward nested	211 bp
	5′-GCTGTCCATATATTTCTCCAG-3′	Reverse nested

Sequences of the primers utilized the detection to the TTSuV1a and TTSuV1b for nested PCR. Bold letters indicate degenerate base. All primers were synthesized by another source (Integrated DNA Technologies, Coralville, Iowa, USA).