Skip to main content
. 2017 Jun 15;9(1):1334503. doi: 10.1080/20002297.2017.1334503

Figure 1.

Figure 1.

Modulation of the expression of microRNAs (mRNAs) encoding cytokine signaling-3 (SOCS-3), interferon regulatory factor-1 (IRF-1), epidermal growth factor (EGF), EGF-receptor (EGFR), and signal transducers and activators of transcription (STAT-3) in human gingival epithelial cells (HGEC) infected with Porphyromonas gingivalis and activated by its lipopolysaccharide (LPS-Pg). (a) HGEC were infected for 2 h with P. gingivalis at different multiplicities of infection (MOI; 10 or 100) and with heat-killed P. gingivalis (Ina) at a MOI of 100. Uninfected cells served as control. (b) HGEC were activated for 2 h and 24 h by 0.5 µg/mL and 1 µg/mL of LPS-Pg. Unactivated cells served as controls. In both experiments, total RNA were prepared, and levels of mRNAs encoding SOCS-3, IRF-1, EGF, EGFR, and STAT-3 were determined by quantitative reverse transcription polymerase chain reaction analysis. All results were presented as the quantity relative to β-actin as a reference gene. Differences (*) between a test mRNA and the control HGEC were analyzed with Student’s t-test (p < 0.0005). All experiments were repeated three times, and the results are expressed as mean ± the standard deviation.