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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: Annu Rev Biophys. 2017 Mar 15;46:199–225. doi: 10.1146/annurev-biophys-062215-011140

Figure 2.

Figure 2

Schematics of human and Tetrahymena telomerase RNP cores illustrating TR secondary structure and known protein components. TR contains three major structural and functional domains: template/pseudoknot (t/PK), stem terminus element (STE), and biogenesis. (a) Human telomerase core RNP includes TERT, TR, and the H/ACA proteins. Human TERT (blue) interacts with the t/PK and the STE [conserved regions 4 and 5 (CR4/5)] (violet). In the t/PK, the template, pseudoknot, and template boundary element (TBE) are highlighted in red, orange, and yellow, respectively. Helical regions are labeled as P (20). The biogenesis domain comprises an H/ACA scaRNA motif that interacts with the H/ACA proteins dyskerin (dark green), NOP10 (light green), NHP2 (gold), GAR1 (pale blue), and TCAB1 (light purple). (b) Tetrahymena telomerase RNP core includes TR, TERT (blue), and p65 (dark green). Domains of the t/PK and STE are colored as in panel a, with the addition of the single-stranded template recognition element (TRE) in orange. The biogenesis domain at the 3′ end of TR is bound by p65; its N-terminal La module binds the TR polyU tail for biogenesis, and the C-terminal xRRM binds stem 4 around the GA bulge for assembly. Abbreviations: nt, nucleotide; RNP, ribonucleoprotein; scaRNA, small Cajal body ribonucleoprotein; TR, telomerase RNA.