Fig. 5. SYNCRIP regulates the myeloid leukemia stem cell gene expression program.
(a) Gene expression heat map of the top 191 upregulated and downregulated genes from RNA-sequencing analysis of MLL-AF9 leukemia cells transduced with control and shRNAs against Syncrip. n=3 biological replicates. (b–i) GSEA analysis showing gene expression signature (b–c) genes enriched in hematopoietic stem cells and leukemic stem cells (d) genes upregulated in myeloid development program enriched in Syncrip-KD cells (e–f) MLL-AF9 directed taget genes and (g) HOXA9-MEIS1 target genes downregulated in Syncrip-KD cells (h–i) enrichment of MSI2 target genes in SYNCRIP regulated genes.