Concentration dependence of the impact of PC (A–C) and TXY536 (D–F) on the polymerization of wild-type (A,D), V307R single mutant (B,E), and V307R/H33E double mutant (C,F) SaFtsZ, as determined by monitoring time-dependent changes in absorbance at 340 nm (A340) at 25 °C. The time-dependent A340 polymerization profiles are shown for each target FtsZ in the presence of DMSO vehicle or the indicated concentrations of PC and TXY536. Experimental conditions were as described in the legend to Figure 2.