fen1-1 is also a suppressor of ros1 at the 35S:NPTII locus but not the RD29A:LUC locus.
(a), (b) fen1-1 de-represses the silencing of 35S: NPTII in the ros1-1 mutant. Phenotype of the transgenic wild type (TWT), ros1-1, and three fen1-1 ros1-1 lines on a MS plate (a) or a MS plate supplied with 50 mg L−1 kanamycin (Kan) (b).
(c), (d) fen1-1 mutants could not release the silencing of RD29A:LUC caused by the ros1 mutant. Luminescence imaging of TWT, ros1-1, fen1-1, and fen1-1 ros1-1 samples. The bright-field image is shown in (c) and the LUC signal is presented in (d). The photos were taken after the treatment of the plants with 300 mM NaCl for 3 h.