Skip to main content
. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Plant J. 2016 Aug 5;87(6):629–640. doi: 10.1111/tpj.13224

Figure 5.

Figure 5

fen1-1 releases NPTII silencing in dms3-4 by decreasing the H3K27me3 level.

(a) Integrated Genome Viewer visualization of the epigenetic profile in H3K27me3 (K27me3), H3K4me3 (K4me3), and H3K9me2 (K9me2) at transgenic loci in the transgenic wild type (TWT) and fen1-1 mutant. (b) H3K27me3 levels at the NPTII gene body were reduced by the FEN1 mutation. Alterations in histone modification among TWT, dms3-4, fen1-1 dms3-4, fen1-1 and two complementary lines were verified by chromatin immunoprecipitation-qPCR. (c), (d) Histone modification status at the control region. UBI was used as a positive control for H3K4me3 and negative control for H3K27me3 and H3K9me2. (e), (f) fen1-1-released NPTII silencing in dms3-4 was not due to decreasing DNA methylation. DNA methylation levels of 35S (e) and NOS (f) were analyzed by bisulfite sequencing.