FEN1 mediates H3K27me3 modification and gene expression.
(a) Histone modification enrichment profiles for the transgenic wild type (TWT) and fen1-1 mutant among 703 genes with reduced H3K27me3 in the fen1-1 mutant. The genes were normalized to the same length [from transcription start site (TSS) to transcription termination site (TTS)] with a 2-kb extension upstream from the TSS or downstream from the TTS, respectively. (b), (c) Heat map and boxplot depicting the expression levels of the genes in (a) obtained from the RNA-seq data. The values are log2(FPKM)-transformed (FPKM, fragments per kilobase of transcript per million fragments mapped). ***P < 0.001, paired Student’s t-test. (d) Histone modification enrichment profiles for TWT and the fen1-1 mutant among 381 genes with an increased H3K27me3 level in the fen1-1 mutant. The genes were normalized to the same length (from TSS to TTS) with a 2-kb extension upstream from the TSS or downstream from the TTS, respectively. (e), (f) Heat map and boxplot depicting the expression of the genes in (d) obtained from the RNA-seq data. The values are log2(FPKM)-transformed. n.s., not statistically significant, paired Student’s t-test.