FEN1 is required for silencing endogenous H3K27me3-marked genes.
(a) Integrated Genome Viewer visualization of the epigenetic profiles and mRNA levels in the affected genes in the fen1-1 mutant. The values were set to the same scale between the transgenic wild type (TWT) and the fen1-1 mutant.
(b)–(d) Histone modification changes of three endogenous genes examined by chromatin immunoprecipitation-qPCR. Values were related to input, bars are mean ± SE (n = 3).
(e) Relative gene expression level of indicated samples detected by qRT-PCR. Values were normalized to the UBI gene. Bars are mean ± SE (n = 3).