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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Plant Cell Environ. 2016 Nov 2;40(1):56–68. doi: 10.1111/pce.12826

Figure 6.

Figure 6

Ribosome-bound mRNA profiles under cold stress. (a) Ribosome-bound mRNA analysis in gel. The extracted polysomes were loaded to 11 mL 20%–50% sucrose gradients and then centrifuged at 175 000 g for 150 min at 4 °C. After centrifugation, 22 fractions (0.5 mL/fraction) were harvested from the top to the bottom of the sucrose gradients. RNA from each fraction was isolated for RNA gel analysis. The 1–6th and 22nd fractions, in which no mRNA accumulated, were removed. (b) Polysome profiles of atgcn1-1 plants. After centrifugation, OD260 values from the bottom of the sucrose gradients were recorded. CON, control condition; Cold24, 24 h of cold treatment. Arrow shows the increased mRNA content in ribosomes enriched in 50% sucrose in atgcn1-1 under cold stress. The highest peak pointed to 50% sucrose should be the interference of chlorophyll, which occurred in the green fractions of sucrose gradients. Experiments were repeated three times with similar results.