The drug was given as detailed in the legend of Fig. 1, and neurobehavioral analyses and body weight measurement were performed at pre- and post-MCAO. The same animals were MRI-scanned at 48 h and at 21 days (data presented in Fig. 1). Delayed MRE-269 treatment significantly improved long-term neurological outcomes assessed by adhesive removal test (A) and rotarod (B) compared to the vehicle group from day 3 to day 21 after MCAO. C) Bar graphs showing body weight loss was continuously increased from 48 h to day 21 after MCAO in the vehicle group, but MRE-269 treatment significantly attenuated body weight loss after one week following MCAO. Data in panels A to C were expressed as mean ± SEM, *P<0.05, **P<0.01 and ***P<0.001 versus vehicle. Vehicle, n=8; MRE-269, n=9.