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. 2017 Jul 12;17:158. doi: 10.1186/s12866-017-1067-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Influence of sigD overexpression on cell cultures and cell morphology. a Cell cultures with 50 μM of IPTG after 36 h of cultivation are shown. b Supernatant turbidity with different concentrations of IPTG (0 μM, 10 μM, 50 μM) after 36 h is shown. Error bars represent standard deviations from biological triplicates. Microscopic images of the WT strain (c) and the sigD overexpressing strain (d) are shown. Cells in the stationary phase were observed under the microscope with a magnification of 1000. e Distribution of the size of cell aggregates is shown. The size of cell aggregates was analyzed by ImageJ, and the distribution was visualized by the box-and-whisker plot. Lower whisker, lower quantile, median, upper quantile and upper whisker are shown. The cross point indicates mean, and outliners were plotted as individual points