Fig 3.

Deacetylation of histone H4 is initiated after the diauxic shift. Immunoblots of yeast extracts probed with antibodies that bind the four conserved N-terminal acetylated lysines in histone H4 (upper) or total histone H4 (lower) over a time course from logarithmic growth, through the diauxic shift (at 14 hours) to 48 hours and then from quiescent (Q) cells purified from a seven day old stationary phase culture. Below each immunoblot is a ponceau S stain of protein in the same lane as a loading control.