Fig 4.

Whi5 and Srl3 mutants can arrest in G1 during the transition to quiescence, but they are required for efficient recovery from quiescence a Flow cytometry of DNA content after seven days in culture shows that all four strains are primarily arrested in G1 with 1N DNA content. b The timing of budding as purified quiescent cells re-enter the cell cycle showing the delay in whi5srl3 mutants. c The timing of budding upon release from G1 arrest by alpha factor is not delayed in the absence of Whi5 and Srl3. Note that wild type quiescent cells require two hours to initiate budding compared to the 30 minutes required for budding after release from alpha factor arrest (see (Miles, et al. 2016) for details.)