Fig. 2.

ATL inhibits cell colony formation and blocks the cell cycle. a: U87 and U251 cells were treated with ATL at the indicated doses for the appropriate time; then, the induced colony formation was analyzed, and the colony formation numbers were calculated. b: U87 and U251 cell cycle analysis was performed using a C6 flow cytometer after 48 h of ATL treatment. c: Cyclin D1 and CDK4, which are cell cycle-related proteins in the G1 phase, were evaluated by Western blotting in the two cell lines. The data are presented as the mean ± SD of three separate experiments (*P < 0.05, **P < 0.01, significant differences between the ATL treatment groups and DMSO vehicle control groups)