Fig. 4.

Fusion of the 01-B526-R19 pAsa5 plasmid with pAsa9 through their ISAS5. a The pAsa5 and pAsa9 fusion involved two ISAS5 (full and hollow red arrows): ISAS5ZA, which is the product of a previous recombination, and pAsa9 sole ISAS5. Features from both plasmids that are found in Fig. 1 are included in the diagram for comprehension purposes, and ISs other than the involved ISAS5s were omitted for clarity. The figure is not to scale. The pAsa5 ZA-pAsa9 plasmid presence was visualized by gel electrophoresis (see Fig. 3b) and the ISAS5 recombination and surrounding sequences were amplified by PCR (1 and 2). b Two long PCRs show the junctions between pAsa5 ZA and pAsa9. The numbers match the amplicon shown in the previous diagram. Product 1 is 9348 pb and Product 2 is 8429 pb. A negative control (−) targeting a TTSS region (Additional file 3) was also included