Fig. 3.

15-oxoETE treatment promotes monocyte recruitment and increases E-selectin expression in vitro. Monocyte recruitment was illustrated by panels A and B. a HUVECs were starved for 6 h and treated with 15-oxoETE (0, 0.5, 1, 2, 4, 8 μmol/L) for 6 h. Images represent the mean of adherent monocytes per microscopic field. Scale bar = 50 μm. b Monocyte adhesion was quantified by manual count, The results were normalized to the number of control cells. c, d, e HUVECs were treated with 15-oxoETE at the indicated concentrations as above for 6 h. VCAM-1, ICAM-1, and E-selectin expression in HUVECs were examined by cell ELISA. Results are represented as fold of 15-oxoETE-treated cells in comparison with control, TNF-α was served as positive control. f Effect of 15-oxoETE on the protein level of E-selectin was determined by western blot. All data are expressed as mean ± SD of three independent experiments. (*P < 0.05, **P < 0.01)