Figure 2. Bar plots of combined STRUCTURE analysis for clusters K = 2 to K = 9 of the investigated R. temporaria breeding pond populations in the study area.

STRUCTURE HARVESTER identified K = 4 as the most meaningful number of clusters. Each vertical bar represents one individual, and the color composition visualizes the probability to belong to one of the K clusters defined by STRUCTURE. P10 was excluded from the analysis due to the different life stage of the samples.