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Single exponential decay of Rhodamine 6G dissolved in methanol. a) Intensity image, size 0.5 × 0.5 mm at 1024 × 1024 pixel acquired within 1.9 s (raw data acquisition time: 1.0 s, see text) b) SP-FLIM image with 3x3 pixel binning for the lifetime map. A region of interest (ROI) was selected to determine the lifetime variation (see text) c) Single exponential decay lifetime fit of a 3x3 binned pixel taking IRF into account. Scale bar 100 µm.
