Fig. 7.

Image captured with the endomicroscopic probe in epi-direction a) of a cryosection of 10 μm thickness of an experimental tumor expressing eGFP that was grown in an incubated chicken egg. Morphology of tumor tissue could be visualized (left). Healthy tissue (dark) could be accurately distinguished from malignant lesions (bright) at the boundary of the tumor (right). b) Investigation of a glioblastoma biopsy (volume tissue) surgically removed from the brain of a human. Stained in-vivo with 5-Ala. Four adjacent images 150 ± 20 μm apart from each other (top left to bottom right) demonstrate a diffuse delineation capability of the device between malignant (top left), a transition area and healthy tissue (bottom right). Scale bars 50 μm, respectively.