Fig. 4.

ex-vivo visOCM imaging of the PTLp cortex in a B6SJL/f1 mouse brain slice. (a) A transmission image of the entire mouse hemisphere was first acquired to locate the desired area (green rectangle), which was then imaged with visOCM (b). The mosaic of part of the PTLp cortex, acquired with visOCM, reveals a variety of cortical structures, such as fibers, cell bodies and vascular entities (en-face view). In the mosaic, mainly two types of cells can be visualized, large cells as shown in (c) and smaller darker cells as pointed by arrowheads in (d). A depth scan of (c) is shown in Visualization 1 ^{(8.5MB, AVI)} . The orthogonal views in (c–d) highlight the three-dimensional repartition of these cell types within the depth of the slice. Capillary vessels can be discriminated from the tissue as either dark or bright structures as shown in (e) and (f) respectively. These different contrasts are further revealed in the orthogonal slices accompanying the close-ups, where one can trace the path of the hollow dark lumen or the bright vessel border, pointed by the arrowheads. En-face images at different depths show that visOCM can perform imaging over >20 μm (g). Scalebars: 150 μm (b), 50 μm for the en-face and 20 μm for the orthogonal views (c–g).