Table 1.

Tm values (50% unfolding) for thermal denaturation were calculated for PLK4 protein. In addition, DTm values measured in the presence of ATP, VX-680, staurosporine and BI2536 are presented. Data are mean ± SD for two independent experiments, each performed in duplicate.

		ΔTm/°C
Protein	Tm/°C	+ ATP:Mn2+	+ 10 μM VX-680	+ 100 μM VX-680	+ 10 μM Staurosporine	+ 100 μM Staurosporine	+ 10 μM BI2536	+ 100 μM BI2536
PLK4 (1-269)	38.03 ± 0.10	2.16 ± 0.23	6.06 ± 0.27	12.55 ± 1.74	11.00 ± 0.13	16.46 ± 0.15	-0.94 ± 0.57	-1.09 ± 0.57
PLK4 Y177E	35.82 ± 0.01	2.98 ± 0.11	7.73 ± 0.16	13.62 ± 4.82	12.76 ± 0.33	17.79 ± 0.32	-0.82 ± 0.57	-0.75 ± 0.19
PLK4 Y177F	36.06 ± 0.07	2.81 ± 0.04	8.25 ± 0.76	15.94 ± 0.13	13.37 ± 0.95	18.14 ± 0.68	0.36 ± 1.36	-0.37 ± 0 .82
PLK4 D154A	37.49 ± 0.26	0.37 ± 0.16	8.73 ± 0.06	14.13 ± 0.41	11.97 ± 0.02	17.50 ± 0.18	0.45 ± 0.22	-0.20 ± 0.9