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. 2017 Jun 5;97(1):30–37. doi: 10.4269/ajtmh.15-0771

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 1. — Humoral immune response upon the different treatments, as measured in sera from immunized mice. 47-kDa outer membrane protein–specific immunoglobulins including total (A) IgG, (B) IgG1, (C) IgG2a, and (D) IgA were measured by indirect enzyme-linked immunosorbent assay after every immunization. Statistically significant differences between the PBS control group and each recombinant protein immunization group are indicated with * (P < 0.05) or ** (P < 0.01). Statistically significant differences between the pVAX1 control group and each DNA vaccination group are indicated with † (P < 0.05) or †† (P < 0.01).